Bl tabelle 2019/16

bl tabelle 2019/16

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Bl Tabelle 2019/16 Video

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Histopathology , the microscopic study of diseased tissue, is an important tool in anatomical pathology , since accurate diagnosis of cancer and other diseases usually requires histopathological examination of samples.

Trained physicians, frequently licensed pathologists , are the personnel who perform histopathological examination and provide diagnostic information based on their observations.

The trained personnel who prepare histological specimens for examination are histotechnicians, histotechnologists, histology technicians HT , histology technologists HTL , medical scientists, medical laboratory technicians , or biomedical scientists , and their support workers.

Their field of study is called histotechnology. In the 17th century, Italian Marcello Malpighi invented one of the first microscopes for studying tiny biological entities.

Malpighi analysed several parts of the organs of bats, frogs and other animals under the microscope. Malpighi, while studying the structure of the lung, noticed its membranous alveoli and the hair-like connections between veins and arteries, which he named capillaries.

His discovery established how the oxygen breathed in, enters the blood stream and serves the body. In the 19th century, histology was an academic discipline in its own right.

The French anatomist Bichat introduced the concept of tissue in anatomy in , and the term "histology" first appeared in a book of Karl Meyer in Bichat described twenty-one human tissues, which can be subsumed under the four categories currently accepted by histologists.

During the 19th century, many fixation techniques were developed by Adolph Hannover solutions of chromates and chromic acid , Franz Schulze and Max Schultze osmic acid , Alexander Butlerov formaldehyde and Benedikt Stilling freezing.

They had conflicting interpretations of the neural structure of the brain based on differing interpretations of the same images.

Cajal won the prize for his correct theory, and Golgi for the silver staining technique he invented to make it possible.

There are four basic types of animal tissues: All tissue types are subtypes of these four basic tissue types for example, blood is classified as connective tissue, since the blood cells are suspended in an extracellular matrix, the plasma.

The tissues from plants, fungi, and microorganisms can also be examined histologically. Their structure is very different from animal tissues.

For plants, the study of their tissues is more commonly called as plant anatomy , with the following main types:.

Chemical fixatives are used to preserve tissue from degradation, and to maintain the structure of the cell and of sub-cellular components such as cell organelles e.

For electron microscopy, the most commonly used fixative is glutaraldehyde , usually as a 2. These fixatives preserve tissues or cells mainly by irreversibly cross-linking proteins.

The main action of these aldehyde fixatives is to cross-link amino groups in proteins through the formation of methylene bridges -CH 2 - , in the case of formaldehyde, or by C 5 H 10 cross-links in the case of glutaraldehyde.

This process, while preserving the structural integrity of the cells and tissue can damage the biological functionality of proteins, particularly enzymes , and can also denature them to a certain extent.

This can be detrimental to certain histological techniques. Further fixatives are often used for electron microscopy such as osmium tetroxide or uranyl acetate.

However, extraction and analysis of nucleic acids and proteins from formalin-fixed, paraffin-embedded tissues is possible using appropriate protocols.

Frozen section procedure is a rapid way to fix and mount histology sections using a refrigeration device called a cryostat.

It is often used after surgical removal of tumors to allow rapid determination of margin that the tumor has been completely removed. The aim of tissue processing is to remove water from tissues and replace with a medium that solidifies to allow thin sections to be cut.

For light microscopy, paraffin wax is most frequently used. Since it is immiscible with water, the main constituent of biological tissue, water must first be removed in the process of dehydration.

Samples are transferred through baths of progressively more concentrated ethanol to remove the water. This is followed by a hydrophobic clearing agent such as xylene to remove the alcohol, and finally molten paraffin wax , the infiltration agent, which replaces the xylene.

Paraffin wax does not provide a sufficiently hard matrix for cutting very thin sections for electron microscopy. Instead, resins are used. Epoxy resins are the most commonly employed embedding media, but acrylic resins are also used, particularly where immunohistochemistry is required.

Again, the immiscibility of most epoxy and acrylic resins with water necessitates the use of dehydration, usually with ethanol.

After the tissues have been dehydrated, cleared, and infiltrated with the embedding material, they are ready for external embedding.

During this process the tissue samples are placed into molds along with liquid embedding material such as agar, gelatine, or wax which is then hardened.

This is achieved by cooling in the case of paraffin wax and heating curing in the case of the epoxy resins.

The acrylic resins are polymerised by heat, ultraviolet light, or chemical catalysts. The hardened blocks containing the tissue samples are then ready to be sectioned.

Because formalin-fixed, paraffin-embedded FFPE tissues may be stored indefinitely at room temperature, and nucleic acids both DNA and RNA may be recovered from them decades after fixation, FFPE tissues are an important resource for historical studies in medicine.

Embedding can also be accomplished using frozen, non-fixed tissue in a water-based medium. Pre-frozen tissues are placed into molds with the liquid embedding material, usually a water-based glycol, OCT, TBS, Cryogel, or resin, which is then frozen to form hardened blocks.

For light microscopy, a steel knife mounted in a microtome is used to cut 4- micrometer -thick tissue sections which are mounted on a glass microscope slide.

For transmission electron microscopy, a diamond knife mounted in an ultramicrotome is used to cut nanometer -thick tissue sections which are mounted on a 3-millimeter-diameter copper grid.

Then the mounted sections are treated with the appropriate stain. Sections can be cut through the tissue in a number of directions. For pathological evaluation of tissues, vertical sectioning, cut perpendicular to the surface of the tissue to produce a cross section is the usual method.

Horizontal also known as transverse or longitudinal sectioning, cut along the long axis of the tissue, is often used in the evaluation of the hair follicles and pilosebaceous units.

Fixed or unfixed tissue may be frozen and sliced using a microtome mounted in a refrigeration device known as a cryostat.

The frozen sections are mounted on a glass slide and may be stained to enhance the contrast between different tissues. Unfixed frozen sections can also be used for studies requiring enzyme localization in tissues and cells.

It is necessary to fix tissue for certain procedures such as antibody linked immunofluorescence staining. Frozen sectioning can also be used to determine if a tumour is malignant when it is found incidentally during surgery on a patient.

Biological tissue has little inherent contrast in either the light or electron microscope. Staining is employed to give both contrast to the tissue as well as highlighting particular features of interest.

Where the underlying mechanistic chemistry of staining is understood, the term histochemistry is used. Hematoxylin, a basic dye, stains nuclei blue due to an affinity to nucleic acids in the cell nucleus; eosin, an acidic dye, stains the cytoplasm pink.

Uranyl acetate and lead citrate are commonly used to impart contrast to tissue in the electron microscope. There are many other staining techniques that have been used to selectively stain cells and cellular components.

One of these techniques involves marking peripheral tumors or surgical margins, in which a certain color of dye is applied to the posterior border of a sample, another to the anterior, etc.

Other compounds used to color tissue sections include safranin , Oil Red O , Congo red , Fast green FCF , silver salts, and numerous natural and artificial dyes that usually originated from the development of dyes for the textile industry.

Histochemistry refers to the science of using chemical reactions between laboratory chemicals and components within tissue. A commonly performed histochemical technique is the Perls Prussian blue reaction, used to demonstrate iron deposits in diseases like hemochromatosis.

Histology samples have often been examined by radioactive techniques. In historadiography , a slide sometimes stained histochemically is X-rayed.

More commonly, autoradiography is used to visualize the locations to which a radioactive substance has been transported within the body, such as cells in S phase undergoing DNA replication which incorporate tritiated thymidine , or sites to which radiolabeled nucleic acid probes bind in in situ hybridization.

For autoradiography on a microscopic level, the slide is typically dipped into liquid nuclear tract emulsion, which dries to form the exposure film.

Individual silver grains in the film are visualized with dark field microscopy. Recently, antibodies have been used to specifically visualize proteins, carbohydrates, and lipids.

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Processing commonly leads to changes like shrinkage, washing migthy of particular cellular components, color changes in different tissues types and alterations of the structures in the tissue. Unsourced material may be challenged and removed. Trained physicians, frequently licensed pathologistsare the personnel who perform histopathological examination and provide diagnostic information based on their observations. The aim of tissue processing is to remove water from tissues and replace with a medium that solidifies to allow thin sections to be cut. There are four basic types of animal tissues: Since it is immiscible with water, beliebte iphone spiele main constituent of biological tissue, water must first be removed in the process of dehydration. We move on and keep fighting in our next 17 finals Tonybet email A commonly performed histochemical technique is the Perls Prussian blue reaction, used to demonstrate iron deposits in diseases like hemochromatosis. Individual silver bl tabelle 2019/16 in the film are visualized with dark field microscopy. Views Read Casino usa alter View history.

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The main action of these aldehyde fixatives is to cross-link amino groups in proteins through the formation of methylene bridges -CH 2 - , in the case of formaldehyde, or by C 5 H 10 cross-links in the case of glutaraldehyde.

This process, while preserving the structural integrity of the cells and tissue can damage the biological functionality of proteins, particularly enzymes , and can also denature them to a certain extent.

This can be detrimental to certain histological techniques. Further fixatives are often used for electron microscopy such as osmium tetroxide or uranyl acetate.

However, extraction and analysis of nucleic acids and proteins from formalin-fixed, paraffin-embedded tissues is possible using appropriate protocols.

Frozen section procedure is a rapid way to fix and mount histology sections using a refrigeration device called a cryostat.

It is often used after surgical removal of tumors to allow rapid determination of margin that the tumor has been completely removed.

The aim of tissue processing is to remove water from tissues and replace with a medium that solidifies to allow thin sections to be cut.

For light microscopy, paraffin wax is most frequently used. Since it is immiscible with water, the main constituent of biological tissue, water must first be removed in the process of dehydration.

Samples are transferred through baths of progressively more concentrated ethanol to remove the water. This is followed by a hydrophobic clearing agent such as xylene to remove the alcohol, and finally molten paraffin wax , the infiltration agent, which replaces the xylene.

Paraffin wax does not provide a sufficiently hard matrix for cutting very thin sections for electron microscopy. Instead, resins are used. Epoxy resins are the most commonly employed embedding media, but acrylic resins are also used, particularly where immunohistochemistry is required.

Again, the immiscibility of most epoxy and acrylic resins with water necessitates the use of dehydration, usually with ethanol. After the tissues have been dehydrated, cleared, and infiltrated with the embedding material, they are ready for external embedding.

During this process the tissue samples are placed into molds along with liquid embedding material such as agar, gelatine, or wax which is then hardened.

This is achieved by cooling in the case of paraffin wax and heating curing in the case of the epoxy resins. The acrylic resins are polymerised by heat, ultraviolet light, or chemical catalysts.

The hardened blocks containing the tissue samples are then ready to be sectioned. Because formalin-fixed, paraffin-embedded FFPE tissues may be stored indefinitely at room temperature, and nucleic acids both DNA and RNA may be recovered from them decades after fixation, FFPE tissues are an important resource for historical studies in medicine.

Embedding can also be accomplished using frozen, non-fixed tissue in a water-based medium. Pre-frozen tissues are placed into molds with the liquid embedding material, usually a water-based glycol, OCT, TBS, Cryogel, or resin, which is then frozen to form hardened blocks.

For light microscopy, a steel knife mounted in a microtome is used to cut 4- micrometer -thick tissue sections which are mounted on a glass microscope slide.

For transmission electron microscopy, a diamond knife mounted in an ultramicrotome is used to cut nanometer -thick tissue sections which are mounted on a 3-millimeter-diameter copper grid.

Then the mounted sections are treated with the appropriate stain. Sections can be cut through the tissue in a number of directions.

For pathological evaluation of tissues, vertical sectioning, cut perpendicular to the surface of the tissue to produce a cross section is the usual method.

Horizontal also known as transverse or longitudinal sectioning, cut along the long axis of the tissue, is often used in the evaluation of the hair follicles and pilosebaceous units.

Fixed or unfixed tissue may be frozen and sliced using a microtome mounted in a refrigeration device known as a cryostat. The frozen sections are mounted on a glass slide and may be stained to enhance the contrast between different tissues.

Unfixed frozen sections can also be used for studies requiring enzyme localization in tissues and cells. It is necessary to fix tissue for certain procedures such as antibody linked immunofluorescence staining.

Frozen sectioning can also be used to determine if a tumour is malignant when it is found incidentally during surgery on a patient.

Biological tissue has little inherent contrast in either the light or electron microscope. Staining is employed to give both contrast to the tissue as well as highlighting particular features of interest.

Where the underlying mechanistic chemistry of staining is understood, the term histochemistry is used. Hematoxylin, a basic dye, stains nuclei blue due to an affinity to nucleic acids in the cell nucleus; eosin, an acidic dye, stains the cytoplasm pink.

Uranyl acetate and lead citrate are commonly used to impart contrast to tissue in the electron microscope. There are many other staining techniques that have been used to selectively stain cells and cellular components.

One of these techniques involves marking peripheral tumors or surgical margins, in which a certain color of dye is applied to the posterior border of a sample, another to the anterior, etc.

Other compounds used to color tissue sections include safranin , Oil Red O , Congo red , Fast green FCF , silver salts, and numerous natural and artificial dyes that usually originated from the development of dyes for the textile industry.

Histochemistry refers to the science of using chemical reactions between laboratory chemicals and components within tissue.

A commonly performed histochemical technique is the Perls Prussian blue reaction, used to demonstrate iron deposits in diseases like hemochromatosis.

Histology samples have often been examined by radioactive techniques. In historadiography , a slide sometimes stained histochemically is X-rayed.

More commonly, autoradiography is used to visualize the locations to which a radioactive substance has been transported within the body, such as cells in S phase undergoing DNA replication which incorporate tritiated thymidine , or sites to which radiolabeled nucleic acid probes bind in in situ hybridization.

For autoradiography on a microscopic level, the slide is typically dipped into liquid nuclear tract emulsion, which dries to form the exposure film.

Individual silver grains in the film are visualized with dark field microscopy. Recently, antibodies have been used to specifically visualize proteins, carbohydrates, and lipids.

This process is called immunohistochemistry , or when the stain is a fluorescent molecule, immunofluorescence. This technique has greatly increased the ability to identify categories of cells under a microscope.

Other advanced techniques, such as nonradioactive in situ hybridization, can be combined with immunochemistry to identify specific DNA or RNA molecules with fluorescent probes or tags that can be used for immunofluorescence and enzyme-linked fluorescence amplification especially alkaline phosphatase and tyramide signal amplification.

Fluorescence microscopy and confocal microscopy are used to detect fluorescent signals with good intracellular detail.

Digital cameras are increasingly used to capture histological and histopathological image. A Text and Atlas. Plastic embedding is commonly used in the preparation of material for electron microscopy.

Tissues are embedded in epoxy resin. Very thin sections less than 0. The sections are stained with electron dense stains uranium and lead so that they can be seen with the electron microscope.

Artifacts are structures or features in tissue that interfere with normal histological examination. These are not always present in normal tissue and can come from outside sources.

Artifacts interfere with histology by changing the tissues appearance and hiding structures. These can be divided into two categories:.

These are features and structures that have been introduced prior to the collection of the tissues. A common example of these include: Artifacts can result from tissue processing.

Processing commonly leads to changes like shrinkage, washing out of particular cellular components, color changes in different tissues types and alterations of the structures in the tissue.

Because these are caused in a laboratory the majority of post histology artifacts can be avoided or removed after being discovered.

Normal patterning of tissues and artifacts resulting from the tissue preparation process ensure that each histological section is unique. Like a piece of biological art these images provide a deep insight into the organization and function of our bodies.

Histological patterns that look like everyday objects or features are emerging on social and scientific communities [15] and even in histopathology journal articles.

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